Resazurin-Based Assay for Quantifying Living Cells during Alkaline Phosphatase (ALP) Release
نویسندگان
چکیده
منابع مشابه
Evaluation of Alkaline phosphatase (ALP) and Heat Stable- ALP (HS-ALP) in Chronic Obstructive Pulmonary Disease (COPD)
Background: COPD (Chronic Obstructive Pulmonary Disease) is defined as a disease state characterized by airflow limitation that is not fully reversible. The airflow limitation is usually both progressive and associated with an abnormal inflammatory response of the lungs to noxious particles and gases. Tobacco smoke remains the main etiological agent. COPD inflammation initiated by tobacco smoke...
متن کاملAn Alkaline Phosphatase Reporter Gene Assay for Induction of CYP3A4 In Vitro
CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...
متن کاملAn Alkaline Phosphatase Reporter Gene Assay for Induction of CYP3A4 In Vitro
CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...
متن کاملResazurin-based assay for screening bacteria for radiation sensitivity
We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in ...
متن کاملSimple resazurin-based microplate assay for measuring Chlamydia infections.
The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Sciences
سال: 2020
ISSN: 2076-3417
DOI: 10.3390/app10113840